Metagenomics Data Analysis

Metagenomics Data Analysis Introduction
Workflow
Turn-around Time
Publications
FAQ

Introduction

Metagenomics involves study of community microbial genomes without culturing and isolating individual species. Metagenomics Sequencing obtains genomic information directly from microbial communities in their natural habitats. There are two general experimental approaches in Metagenomics Sequencing: one is to sequence the entire genomic DNA sample; the other is to selectively amplify universal and unique regions in these genomes (e.g., 16S rDNA, 18S rDNA) and then sequence the amplified products. Only with next-generation sequencing (deep coverage at practical cost) is it widely feasible to study tens of thousands of microbial genomes together at the same time.

Workflow

Following is a list of common analysis items for Metagenomics Sequencing. One of our expert bioinformaticians will work closely with you to identify a custom analysis workflow most appropriate for your project.

1) Experiment design consultation
2) Data QC and clean up
3) Sequence assembly
4) Biodiversity analysis and binning
5) Gene predication and functional annotation
6) Gene Ontology and pathway analysis
7) Phylogenetic analysis
8) Written project report with analysis methods, publication-ready graphics, and references

Turn-around Time

Upon data receipt, we usually finish a typical Metagenomics analysis project in 3-5 days. The actual turn-around time, however, is highly dependent on sample number, data amount, and project complexity.

Publications

Publications below are representative research or review papers that will help you understand how Metagenomics is employed in biomedical research.

  • Qin, J. et al. (2010) A human gut microbial gene catalogue established by metagenomic sequencing. Nature. 464(7285):59-65.
  • Petrosino, JF. (2009) Metagenomic pyrosequencing and microbial identification. Clin Chem. 55(5):856-66.

FAQ

Contact us to submit a question regarding your Megagenomics Sequencing project.