Methylation Sequencing Data Analysis

Methylation Sequencing Data Analysis Introduction
Workflow
Turn-around Time
Publications
FAQ

Introduction

DNA methylation is an epigenetic regulatory mechanism and plays essential roles in physiology and disease processes. Next-generation sequencing enables genome-wide methylation profiling at single base pair resolution and with accurate quantitation.

There are in general two experimental approaches in monitoring the DNA methylation status: one is based on bisulfite conversation (e.g., whole genome bisulfite sequencing) and the other is based on methylated DNA enrichment (e.g., reduced representation bisulfite sequencing, MeDIP-Seq ).

Workflow

Following is a list of common analysis items for Methylation Sequencing. One of our expert bioinformaticians will work closely with you to identify a custom analysis workflow most appropriate for your project.

1) Experiment design consultation
2) Data QC and clean up
3) Methylation state-sensitive mapping
4) Methylation site calling
5) Gene assignment and methylation site annotation
6) Gene Ontology and pathway analysis
7) Written project report with analysis methods, publication-ready graphics, and references

Turn-around Time

Upon data receipt, we usually finish a typical NGS Methylation Sequencing analysis project in 3-5 days. The actual turn-around time, however, is highly dependent on sample number, data amount, and project complexity.

Publications

Publications below are representative research or review papers that will help you understand how NGS Methylation Sequencing is employed in biomedical research.

  • Kim, JH. et al. (2011) Deep sequencing reveals distinct patterns of DNA methylation in prostate cancer. Genome Res. 21(7):1028-41.
  • Brunner, AL. et al. (2009) Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver. Genome Res. 19(6):1044-56.

FAQ

Contact us to submit a question regarding your Megagenomics Sequencing project.